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KMID : 0613820020120010043
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Journal of Life Science
2002 Volume.12 No. 1 p.43 ~ p.48
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The Optimal Conditions for Fibrinolytic Enzyme Production from Streptomyces sp. JK-20
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Jeong Yong-Kee
Jun Hong-Ki
Kim You-Jung
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Abstract
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An actinomycetes which produces fibrinolytic enzyme was isolated from soil. Characteristics of the isolated strain and the optimal conditions for the productions of fibrinolytic enzyme were summarized as follows; The fibrinolytic enzyme production strain generates gray airmycelium and had about 0.6∼0.8×0.4∼0.8㎛ cylindrical spore, smooth surface and formed spore chain of 10∼40 spores. We have identified this strain as Streptomyces sp. JK-20. This strain was able to grow up at 20∼32℃ and its optimum growth temperature and pH was 24℃ and pH 6.0, respectively. The optimal conditions for porducing fibrinolytic enzyme; carbon source, nitrogen source, metal ions and phosphorous sources was 1% xylose, 0.5% yeast extract, 0.5% polypepton, 0.1% MgSO4.7H2O and 0.1% NaH2PO4.2H2O, respectively. This strain showed the highest productivity of fibrinolytic enzyme after the fourth day under such optimal culture conditions.
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KEYWORD
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fibrinolytic enzyme, Streptomyces sp
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